Reporter

Part:BBa_K1970002:Design

Designed by: Eivind Bøe Drejer   Group: iGEM16_NTNU_Trondheim   (2016-10-14)


Red fluorescent protein controlled by CI-repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1586
    Illegal AgeI site found at 1698
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The most challenging part was choosing the right ribosomal binding site and transcription terminator for the desired expression strength of the red fluorescent protein.


Source

Constructed from iGEM biobricks BBa_R0051, BBa_J06702 and synthesized CI protein gene, ribosomal binding site and transcription terminator.

References